Professional detection and calibration of micropipette
The pipette has a large workload, and long-term use will deform the spring elasticity. In addition, it is plastic and is not resistant to friction, and errors will occur. In order to ensure the accuracy of the pipette transfer liquid volume, the pipette must be regularly calibrated. In order to better play the role of this kind of measuring instruments, the newly purchased instruments must be calibrated before they can be used; for the measuring instruments that are being used, due to long-term use, they will cause errors in the value displayed on the measuring object. Controlling and correcting in time will definitely affect scientific research. Therefore, the pipette used must be regularly verified, corrected, and archived. Only in this way can it be better used in daily work.
At present, the routine testing performed in many laboratory conditions cannot completely replace professional calibration work, because calibration has high technical requirements for the external environment, working conditions and precision instruments used, and these conditions are often in most laboratories. Unavailable. At present, some large pipette manufacturers use globally uniform pipette standard operating specifications, use professional software calibration systems, and online control of analytical balances through computers. Measurement, data collection, calculation, and result evaluation are controlled by software. After completion, all man-made operations are printed out with the computer records and reports. The computer is used to evaluate and certify the data, completely eliminating the possibility of manipulating the calibration results, and appointing local agents to provide professional calibration and maintenance services. The following takes Eppendorf as an example to introduce its professional calibration operation.
(1) Basic operating conditions for calibration
Operation room: independent room, showing temperature and humidity status.
Temperature control: 15-30 ~ C (± 0.5 ℃).
Humidity control: 60% ～ 90%.
Worktop: Shockproof, dustproof, away from heat sources, and no direct sunlight.
Balance: 0.0001g precision analytical balance (5 digits after the decimal point), which needs to be calibrated by the manufacturer every year.
Anti-evaporation device: Humidity trap provided by Eppendorf to prevent the evaporation of weighing liquid.
Test medium: double-distilled water, replaced every 4h, batch replacement cycle is not more than 2 weeks.
(2) Operation process
1) Isothermal treatment: Before calibration, all pipettes and calibration media (table, balance, double-distilled water, etc.) are placed in the same operating room for 4 hours to ensure that they have the same temperature.
2) Internal and external cleaning.
3) Lubricate the piston.
4) Calibration: The three-point calibration method is adopted, that is, according to the range of the pipette, the three points of the lowest range, the middle value and the highest range are selected and tested 10 times, and the average value of each test point is taken to calculate the accuracy Accuracy (precision), the evaluation standard meets the requirements of DIN 12650.
5) Calibration report: A standard report printed by a computer or a PICASO correction report can be provided according to customer needs, in compliance with ISO, DIN or ASTM related standards.
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