Triiodothyronine release-free kit instructions, T3 thyroxine release-free kit instructions

Triiodothyronine release-free kit instructions, T3 thyroxine release-free kit instructions
T3 (HY-001) RIA KI

Triiodothyronine (T3) has a molecular weight of 651. In addition to being secreted by the thyroid, T3 can also be derived from T4 by removing an iodine atom through the action of deiodinase. T3 content is 50-70 times smaller than T4 in circulation. The content of free T3 is only 2-5 times smaller than that of free T4, but the biological activity of T3 is 10 times stronger than that of T4, so it is considered that the biological activity of T3 is more important than T4. The secretion of T3 is also regulated by the negative feedback mechanism of the thalamus-pituitary-thyroid axis. Like T4 in the blood circulation, most of it binds to thyroxine-binding globulin, thyroxine-binding prealbumin and albumin, but T3 binds thyroxine Protein and other affinities are lower than T4. Generally speaking, the changes of T3 content and T4 in blood circulation are parallel in clinical practice, but in some cases, the changes of the two are not consistent. The comprehensive clinical situation of T3 and T4 content is as follows:-3 normal, T4 normal: 1. normal people; 2. subclinical hypothyroidism. T4 increased, T3 increased: 1, hyperthyroidism, 2. Thyroglobulin binding increased; 3, T4 over-treatment. Reduced T4 and reduced T3: 1. Decreased thyroid function; 2. Decreased thyroxine-binding globulin binding capacity. T4 decreased, T3 normal: mildly decreased thyroid function. T4 is normal, T3 is elevated: 1. T3 toxemia; 2, T3 overtreatment; 3. Early after treatment of hyperthyroidism; 4. Early stage of recurrence of hyperthyroidism; 5. Exophthalmic hyperthyroidism (compensatory Elevated); 6. Autocrine nodular goiter; 7. Endemic goiter (compensated increase); 8. Subclinical hypothyroidism (compensated increase); . The content of T3 rises significantly within a few hours after the birth of the newborn, and maintains the range above the adult level before puberty. In adult males, the T3 content decreases slightly with age, and T3 increases significantly during female pregnancy, reaching a peak before labor. After the surgery, the T3 content dropped by more than 50%. The physiological role of T3 is the same as T4.

Triiodothyronine release kit kit instructions, T3 thyroxine release kit kit composition:
1. T3 mark (red) 1 bottle.
2. One bottle of T3 antibody (blue).
3. One bottle of separating agent (second antibody).
4. 6 bottles of T3 standard products. Their concentrations are: 0, 0.5, 1, 2, 4, 8 ng / ml (ie 0, 0.77, 1.54, 3.08, 6.16, 12.32 nmol / L)
The above reagents should be used evenly.
Method of operation:
1. Sample processing: take blood from a fasting vein in the morning and separate the serum. (2-8) It can be stored for 7 days at ℃. If it is frozen, it can be stored for four weeks. Lipemia or hemolysis samples affect the measurement results.
2. Experimental method: test tube number, operate according to the following table:

Operation table (Unit: ul)
Tube type Standard tube Sample Antiserum Distilled water 125I-T3 Mix well, incubate at 37 ℃ for 1 hour, take three tubes to measure total T (CPM) separating agent
NSB tube 50 (S0)--100 200 500
S0 tube 50-100-200 500
S1-S end tube 50-100-200 500
Sample tube-50 100-200 500

Mix well, leave at room temperature for 15 minutes, centrifuge (3500 rpm) for 15 minutes, aspirate the supernatant, and measure the radioactivity count (CPM) of each tube precipitate.

Triiodothyronine release and exemption kit instruction manual, T3 thyroxine release and exemption kit instruction manual Data processing:
1. Computer processing: It is recommended to use cubic polynomial or log ～ logit method for the function model.
2. Calculation and drawing methods:
Calculate the percent binding rate of each tube: Take S0 (cpm number) as B0 and each standard tube Si (cpm number) as Bi, find the percent binding rate of each tube.

Bi Bi tube (cpm) —NSB tube (cpm)
—% = ———————————————— × 100
B0 B0 tube (cpm) —NSB tube (cpm)

(1) Linear regression: use logX ～ logitY regression to find r, a, b values, and the sample content can be calculated by the following formula.

-a + logit Y
Log X = ————
b
(2) Drawing method: Using the results of each standard point calculation, draw a Bi / B0 ~ logX standard curve graph on semi-logarithmic graph paper or a standard curve graph on log ～ logit graph paper. The unknown sample concentration can be calculated according to Bi / The percentage binding rate of B0 was found from the standard curve diagram.

Normal reference value:
Each laboratory should establish its own normal value. The following normal reference values ​​are for reference only:
T3 (n = 135): (0.8 ～ 2.2) ng / mL (1.23 ~ 3.38nmol / L)

Technical characteristics:
1. Curve range: 0.5 ～ 8.0ng / Ml (0.77 ～ 12.32 nmol / L)
2. Sensitivity: <0.25ng / mL (0.39 nmol / L)
3. Specificity: The cross reaction between T3 antiserum and T4 is qualified.
4. Precision: CVw within the batch <10%, CVb between batches <15%
5. Unit conversion: (nmol / L) = (ng / Ml) × 1.54

Galvanized Barbed Wire

Galvanized Barbed Wire

Galvanized Razor Barbed Wire is made by high quality galvanized wire , it's widely used as protecting wire fence in many fields , such as in national line , railway , high way , airport etc .

CQ is a professional manufacturer of Gabion and Gabion Mattress and have passed ISO 9001:2008 QMS in 2014. Our Gabion and Gabion Mattress can reach or exceed ASTM A975 or EN 10223-3 Standard and gain high reputation from worldwide clients!

Galvanized Barbed Wire,Electro Barbed Wire,Concertina Barbed Wire,Electro Razor Barbed Wire

Anping Chuangqian Wire Mesh Co., Ltd , https://www.apcqwiremesh.com